Rapid Fluorescent-Antibody Stain Technique with Group A Streptococci

Rapid fluorescent-antibody method with bromelase for identification of group A streptococci.

This is a preliminary report on a rapid fluorescent-antibody staining method utilizing the enzyme Bromelase that effectively eliminates nonspecific staining of staphylococci. By this simple method, group A streptococci were accurately identified from 2- to 4-h broth cultures.

Rapid fluorescent-antibody staining technique.

The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Rapid detection of Pneumocystis carinii using a direct fluorescent monoclonal antibody stain.

A collaborative study was undertaken at two institutions to assess the performance of a direct fluorescent-antibody stain for the detection of Pneumocystis carinii in respiratory specimens from patients with known or suspected human immunodeficiency virus type 1 infections. A total of 163 specimens (125 induced sputa, 37 bronchoalveolar lavage fluids, and 1 tracheal aspirate) from 124 patients ...

Rapid detection and identification of Streptococcus iniae using a monoclonal antibody-based indirect fluorescent antibody technique

Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. The traditional plate culture technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations of S. iniae isolates. The rapid transmission and severe economic impact of this disease necessita...